This guide page will take you through how to get your site running in Calibre.
As long as your site is available on the public internet, Calibre can test it — you'll just need a URL. If your site uses form based authentication, Calibre drives the browser to log into your site / application and start testing pages from there. If you have any questions about authentication, please check the authentication guide.
Once you've created your site, Calibre will analyse your site 5 times in order to get some data flowing through. While this is happening (it'll take a minute or two), its a great idea to start adding additional pages.
If your site is a content heavy site, it can be a good idea to test pages that use distinctly different "templates".